The biochemistry and molecular facility offers strategies design for recombinant protein expression.
The gene of interest is cloned into an expression vector, then the optimal expression system and conditions are selected before expressing the protein in E. coli or mammalian cells. We can produce E. coli biomass up to 5L in bioreactor for high density culture. Purification is carried out using chromatographic techniques. Quality control to assess purity and yield is carried out mainly by SDS-PAGE and UV/Vis measurement.
Equipment, ressources and training are also provided for the production and purification of recombinant proteins.
For any analytical experiment with assistance, or for a R&D project, please ask the facility engineers for a detailed quotation.
Laure Bataille l.bataille@iecb.u-bordeaux.fr
Jean-Michel Blanc jm.blanc@iecb.u-bordeaux.fr
Antoine Loquet a.loquet@iecb.u-bordeaux.fr
Brice Kauffmann b.kauffmann@iecb.u-bordeaux.fr
Bournonville Céline, Mori Kentaro, Deslous Paul, Decros Guillaume, Blomeier Tim, Mauxion Jean-Philippe, Jorly Joana, Gadin Stéphanie, Cassan Cédric, Maucourt Mickael, Just Daniel, Brès Cécile, Rothan Christophe, Ferrand Carine, Fernandez-Lochu Lucie, Bataille Laure, Miura Kenji, Beven Laure, Zurbriggen Matias D, Pétriacq Pierre, Gibon Yves, Baldet Pierre
Paiva Telmo O, Schneider Angela N, Bataille Laure, Chovin Arnaud, Anne Agnès, Michon Thierry, Wege Christina, Demaille Christophe
Nottelet Pierre, Bataille Laure, Gourgues Geraldine, Anger Robin, Lartigue Carole, Sirand-Pugnet Pascal, Marza Esther, Fronzes Remi, Arfi Yonathan