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Molecular biology

Services and expertise

    Cloning

    Two cloning methods are proposed : T4 DNA ligase or “In-Fusion Advantage PCR Cloning Kit” Clontech

    Genotyping

    This test allows the differentiation between homozygous or heterozygous animals for a gene of interest. This technique is performed on blood samples and is used for the genotyping in the FTA technical of Wathman

    Site-Directed mutagenesis

    It consists in introducing a specific mutation or deletion in a target gene. Two different PCR methods are used : high fidelity Taq polymerase or Lightning Quick Change mutagenesis kit from Stratagene.

     

    Equipment

    Thermocycler:

    • Mastercycler Pro (Eppendorf).
    • Microvolume or cuvette determination: nanophotometer (Serlabo)

     

      Technical contact

      This e-mail address is being protected from spambots. You need JavaScript enabled to view it , Tel : +33(0)5 4000 6406 / Fax : +33(0)5 4000 6518

       

      Scientific expertise

      This e-mail address is being protected from spambots. You need JavaScript enabled to view it , U 869, IECB

       
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      Institut Européen de Chimie et Biologie
      2, Rue Robert Escarpit - 33607 PESSAC - France
      Tel. : +33 (5) 40 00 30 38 - Fax. : +33 (5) 40 00 30 68
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