Services and expertise

Cloning

Two cloning methods are proposed : T4 DNA ligase or “In-Fusion Advantage PCR Cloning Kit” Clontech

Genotyping

This test allows the differentiation between homozygous or heterozygous animals for a gene of interest. This technique is performed on blood samples and is used for the genotyping in the FTA technical of Wathman

Site-Directed mutagenesis

It consists in introducing a specific mutation or deletion in a target gene. Two different PCR methods are used : high fidelity Taq polymerase or Lightning Quick Change mutagenesis kit from Stratagene.

Equipment

Thermocycler:

• Mastercycler Pro (Eppendorf).

• Microvolume or cuvette determination: nanophotometer (Serlabo)

Technical contact

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Scientific expertise

This e-mail address is being protected from spambots. You need JavaScript enabled to view it , U 869, IECB